./tblastn -num_threads 16 -out /Users/admin/Dropbox/Cluster/BLAST/output/SP_sigeneae_v8_node2_blastout -query /Users/admin/Dropbox/Cluster/BLAST/query/uniprot_sprot -db /Users/admin/Dropbox/Cluster/BLAST/db/cgigas_all_contigs_v8.fa -outfmt 6 -evalue 1E-20 -max_target_seqs 20
Seems a little flawed.
Lets take same approach with Limpet
./tblastn -num_threads 16 -out /Users/admin/Dropbox/Cluster/BLAST/output/Lotgi1_sigeneae_v8_node2_blastout -query /Users/admin/Dropbox/Cluster/BLAST/query/Lotgi1_GeneModels_FilteredModels1_aa.fasta -db /Users/admin/Dropbox/Cluster/BLAST/db/cgigas_all_contigs_v8.fa -outfmt 6 -evalue 1E-10 -max_target_seqs 1
OUTPUT